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Addgene

pCAG-spCas9-N57
(Plasmid #169920)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169920 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-spCas9
  • Backbone manufacturer
    Addgene # 52962
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpCas9-N57
  • Species
    Synthetic
  • Mutation
    N57 is the PAI domain of SB100X
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-spCas9-N57 was a gift from Zhili Rong (Addgene plasmid # 169920 ; http://n2t.net/addgene:169920 ; RRID:Addgene_169920)

  • For your References section:

    Enhancing site-specific DNA integration by a Cas9 nuclease fused with a DNA donor-binding domain. Ma S, Wang X, Hu Y, Lv J, Liu C, Liao K, Guo X, Wang D, Lin Y, Rong Z. Nucleic Acids Res. 2020 Oct 9;48(18):10590-10601. doi: 10.1093/nar/gkaa779. 10.1093/nar/gkaa779 PubMed 32986839
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