pHR_PGK_antiCD19_H1_Gal4VP64
(Plasmid
#169918)
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PurposeExpression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameantiCD19-Notch1-GL4VP64
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SpeciesH. sapiens (human), Synthetic
- Promoter PGK
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR_PGK_antiCD19_H1_Gal4VP64 was a gift from Zhili Rong (Addgene plasmid # 169918 ; http://n2t.net/addgene:169918 ; RRID:Addgene_169918) -
For your References section:
Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system. Huang H, Zhang X, Lv J, Yang H, Wang X, Ma S, Shao R, Peng X, Lin Y, Rong Z. Protein Cell. 2020 Apr;11(4):299-303. doi: 10.1007/s13238-020-00690-1. 10.1007/s13238-020-00690-1 PubMed 32002793