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pHR_PGK_antiCD19_eZ3_Gal4VP64
(Plasmid #169917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    antiCD19-Notch3-GL4VP64
  • Species
    D. rerio (zebrafish), Synthetic
  • Mutation
    Additional EGF repeat between the extracellular domain between antiCD19 and the Notch core
  • Promoter PGK

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR_PGK_antiCD19_eZ3_Gal4VP64 was a gift from Zhili Rong (Addgene plasmid # 169917 ; http://n2t.net/addgene:169917 ; RRID:Addgene_169917)

  • For your References section:

    Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system. Huang H, Zhang X, Lv J, Yang H, Wang X, Ma S, Shao R, Peng X, Lin Y, Rong Z. Protein Cell. 2020 Apr;11(4):299-303. doi: 10.1007/s13238-020-00690-1. 10.1007/s13238-020-00690-1 PubMed 32002793
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