pRGEN-CMV-VPR-L1-CjCas9 V-D
(Plasmid
#169913)
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PurposeExpression of a VPR-dCjCas9 fusion protein to activate gene expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169913 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRGEN-CMV-CjCas9
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Backbone manufacturerAddgene #89752
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedCjCas9
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Alt namedCjeCas9
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SpeciesCampylobacter jejuni
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MutationD8A, H559A in cjCas9
- Promoter CMV
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Tags
/ Fusion Proteins
- VPR (VP64-p65-Rta) (N terminal on insert)
- 2xSV40NLS (N terminal on insert)
- SV40NLS-HA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRGEN-CMV-VPR-L1-CjCas9 V-D was a gift from Zhili Rong (Addgene plasmid # 169913 ; http://n2t.net/addgene:169913 ; RRID:Addgene_169913) -
For your References section:
MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, Zhao S, Feng J, Huang G, Wan QL, Liu J, Huang H, Luan B, Wang D, Zhao X, Lin Y, Zhou Q, Zhang ZN, Rong Z. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174. 10.1093/nar/gkab174 PubMed 33751124
