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CMV-VPR-S-L1-dCjCas9 MiniCAFE
(Plasmid #169910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MiniCAFE
  • Alt name
    Truncated VPR-dCjCas9
  • Alt name
    Truncated VPR-dCjeCas9
  • Species
    Campylobacter jejuni
  • Mutation
    D8A, HNH-truncation (Δ495–609 aa) in CjCas9
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-VPR-S-L1-dCjCas9 MiniCAFE was a gift from Zhili Rong (Addgene plasmid # 169910 ; http://n2t.net/addgene:169910 ; RRID:Addgene_169910)

  • For your References section:

    MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, Zhao S, Feng J, Huang G, Wan QL, Liu J, Huang H, Luan B, Wang D, Zhao X, Lin Y, Zhou Q, Zhang ZN, Rong Z. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174. 10.1093/nar/gkab174 PubMed 33751124
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