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Addgene

pPIDNB
(Plasmid #169901)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169901 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPIDNB
  • Backbone size (bp) 6520
  • Vector type
    Mammalian Expression ; Aivian Expression
  • Promoter Dox inducible
  • Selectable markers
    mNeonGreen; mScarlet serves as a marker for Dox induction

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use AflII and PstI restriction sites for cloning. This plasmid contains mNeonGreen, which requires a paid license. See pPIDCB for an alternative plasmid, which has replaced the mNeongreen with mClover3 and does not require a paid license.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPIDNB was a gift from Richard Schneider (Addgene plasmid # 169901 ; http://n2t.net/addgene:169901 ; RRID:Addgene_169901)

  • For your References section:

    Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development. Chu D, Nguyen A, Smith SS, Vavrusova Z, Schneider RA. Biol Open. 2020 Oct 6;9(10). pii: bio.055343. doi: 10.1242/bio.055343. 10.1242/bio.055343 PubMed 32917762
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