pPIDCB2
(Plasmid
#169900)
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Purpose(Empty Backbone) Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169900 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPIDCB2
- Backbone size (bp) 6529
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Vector typeMammalian Expression ; Avian expression
- Promoter Dox inducible
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Selectable markersmClover3; mScarlet serves as a marker for Dox induction
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use AflII and PstI restriction sites for cloning. This plasmid is identical to pPIDNB2 except it has replaced the mNeonGreen sequence in pPIDNB2, which requires a paid Allele Biotech license, with mClover3, which doesn't require a paid license.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPIDCB2 was a gift from Richard Schneider (Addgene plasmid # 169900 ; http://n2t.net/addgene:169900 ; RRID:Addgene_169900) -
For your References section:
Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development. Chu D, Nguyen A, Smith SS, Vavrusova Z, Schneider RA. Biol Open. 2020 Oct 6;9(10). pii: bio.055343. doi: 10.1242/bio.055343. 10.1242/bio.055343 PubMed 32917762
Map uploaded by the depositor.
