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Addgene

XP1 Xwnt-1
(Plasmid #16990)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGem2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-1
  • Alt name
    Wnt-1
  • Alt name
    wnt1-A
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2000
  • Entrez Gene
    wnt1.L (a.k.a. XELAEV_18013314mg, Xint-1, Xwnt1, int-1, int1, pxwnt-1, wnt-1, wnt1, wnt1-a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: cDNA from stage 17 lambda gt10 of Melton library.
Antisense probe: cut with Hind III and transcribe with Sp6.
Sense probe: cut with Sph1 and transcribe with T7.

Plasmid History:
Constructed by N. Edelstein and R. Moon on date of 8/92.

Citation: Wolda et al., Developmental Biology 155, (1993).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XP1 Xwnt-1 was a gift from Randall Moon (Addgene plasmid # 16990 ; http://n2t.net/addgene:16990 ; RRID:Addgene_16990)

  • For your References section:

    Overlapping expression of Xwnt-3A and Xwnt-1 in neural tissue of Xenopus laevis embryos. Wolda SL, Moody CJ, Moon RT. Dev Biol. 1993 Jan. 155(1):46-57. 10.1006/dbio.1993.1005 PubMed 8416844
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