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Addgene

pSin4 UAP1 F383G
(Plasmid #169891)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169891 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSin4 EF1a IRES Puro
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UDP-N-acetylhexosamine pyrophosphorylase
  • Alt name
    UAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1518
  • Mutation
    Changed Phenylalanine (F) at position 383 to glycine (G)
  • GenBank ID
    6675
  • Entrez Gene
    UAP1 (a.k.a. AGX, AGX1, AGX2, SPAG2)
  • Tag / Fusion Protein
    • None (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated in the lab a long time ago and we cannot locate detailed cloning information at this point.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSin4 UAP1 F383G was a gift from Jennifer Kohler (Addgene plasmid # 169891 ; http://n2t.net/addgene:169891 ; RRID:Addgene_169891)

  • For your References section:

    Photocrosslinking O-GlcNAcylated Proteins to Neighboring Biomolecules. Capota E, Wu H, Kohler JJ. Curr Protoc. 2021 Jul;1(7):e201. doi: 10.1002/cpz1.201. 10.1002/cpz1.201 PubMed 34288588
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