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Addgene

XP3 Xwnt-4
(Plasmid #16988)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16988 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGem2
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-4
  • Alt name
    Wnt-4
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1800
  • GenBank ID
    U13183
  • Entrez Gene
    wnt4 (a.k.a. Xwnt4, wnt-4, xwnt-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: Xwnt-4 cDNA. Antisense probe cut with Nhe1 and transcribe with T7.
Sense probe cut with Sac1 and transcribe with SP6.

Plasmid History:
Constructed by Lynn McGrew on date of 11/90.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XP3 Xwnt-4 was a gift from Randall Moon (Addgene plasmid # 16988 ; http://n2t.net/addgene:16988 ; RRID:Addgene_16988)
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