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Addgene

XP17 Xwnt-5A
(Plasmid #16985)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-5A
  • Alt name
    Wnt-5A
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    3000
  • Entrez Gene
    wnt5a.L (a.k.a. Xwnt-5A, wnt-5a, wnt5a-a, wnt5a-b)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: cDNA from Melton lambda gt10 oocyte library.

Transcription: linearize with Xba1 and transcribe with SP6.

Plasmid History:
Constructed by Randall Moon on date of 9/15/90.

Notes for Use:
EcoR1 insert of lambda G6 of 2/11/90. Contains significant 5' UTR and is less translatable in vitro per ng RNA than Xwnt-5A Hind III/SP64T. However, the type of activity is the same in both constructs.

Citation: Moon et al., Development 119, 97 (1993)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XP17 Xwnt-5A was a gift from Randall Moon (Addgene plasmid # 16985 ; http://n2t.net/addgene:16985 ; RRID:Addgene_16985)

  • For your References section:

    Xwnt-5A: a maternal Wnt that affects morphogenetic movements after overexpression in embryos of Xenopus laevis. Moon RT, Campbell RM, Christian JL, McGrew LL, Shih J, Fraser S. Development. 1993 Sep. 119(1):97-111. PubMed 8275867
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