pCEP4-myc-S (B.1.351 lineage / South African variant)
(Plasmid #169849)

• Purpose: Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag
• Depositing Lab: Erik Procko
• Publication: Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. doi: 10.1038/s41589-021-00965-6. Epub 2022 Jan 19.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10381
• Total vector size (bp): 14263
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Protein S
• Alt name: spike
• Species: SARS coronavirus 2
• Insert Size (bp): 3882
• Mutation: Codon-optimized for human cell expression.
• GenBank ID:
• Promoter: CMV
• Tag / Fusion Protein:
  • HA leader and c-myc tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: EBV reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Haisheng Yu (Chinese Academy of Medical Sciences)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry