Skip to main content
Addgene

pCEP4-myc-S (B.1.1.7 lineage / UK variant)
(Plasmid #169848)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169848 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10381
  • Total vector size (bp) 14263
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protein S
  • Alt name
    spike
  • Species
    SARS coronavirus 2
  • Insert Size (bp)
    3882
  • Mutation
    Codon-optimized for human cell expression.
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • HA leader and c-myc tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer EBV reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Haisheng Yu (Chinese Academy of Medical Sciences)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-myc-S (B.1.1.7 lineage / UK variant) was a gift from Erik Procko (Addgene plasmid # 169848 ; http://n2t.net/addgene:169848 ; RRID:Addgene_169848)

  • For your References section:

    Engineered ACE2 decoy mitigates lung injury and death induced by SARS-CoV-2 variants. Zhang L, Dutta S, Xiong S, Chan M, Chan KK, Fan TM, Bailey KL, Lindeblad M, Cooper LM, Rong L, Gugliuzza AF, Shukla D, Procko E, Rehman J, Malik AB. Nat Chem Biol. 2022 Mar;18(3):342-351. doi: 10.1038/s41589-021-00965-6. Epub 2022 Jan 19. 10.1038/s41589-021-00965-6 PubMed 35046611
Related items: