pMXS-IRES-BLAST flag-CAD M1601E
(Plasmid #169828)

• Purpose: Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain
• Depositing Lab: Richard Possemato
• Publication: Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.

Backbone

• Vector backbone: pMXS-IRES-BLAST
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 12300
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAD
• Alt name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• Species: H. sapiens (human)
• Insert Size (bp): 6700
• Mutation: M1601E; TCCC -> AGTC silent mutations at nt527-530 eliminating PAM site for sgRNA target sequence 5’-ATTGGGGTCCAAGAATGGCA-3’
• GenBank ID: BC065510.1
• Tag / Fusion Protein:
  • Flag tag (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pMXS-IRES-BLAST flag-CAD M1601E

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry