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Addgene

pDU1
(Plasmid #169817)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169817 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX458
  • Total vector size (bp) 9567
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tCD19
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1002
  • Promoter Cbh
  • Tag / Fusion Protein
    • fusion protein (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TTTATGGCGAGGCGGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDU1 was a gift from Andrea Rossi (Addgene plasmid # 169817 ; http://n2t.net/addgene:169817 ; RRID:Addgene_169817)

  • For your References section:

    Fast but not furious: a streamlined selection method for genome-edited cells. Ramachandran H, Martins S, Kontarakis Z, Krutmann J, Rossi A. Life Sci Alliance. 2021 Apr 26;4(6). pii: 4/6/e202101051. doi: 10.26508/lsa.202101051. Print 2021 Jun. 10.26508/lsa.202101051 PubMed 33903218
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