pBS-ACTB-C200-GFPfr1
(Plasmid #169798)

• Purpose: THe donor vector for the ACTB gene locus.
• Depositing Lab: Natsuko Chiba
• Publication: Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1038/s41598-018-38311-x.

Backbone

• Vector backbone: pBlueScript II SK (+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2959
• Total vector size (bp): 3383
• Modifications to backbone: None
• Vector type: HR donor vector for human ACTB gene.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Donor sequence to ACTB gene for HR
• Species: H. sapiens (human)
• Insert Size (bp): 424
• Mutation: Partial sequence of GFP is inserted before the stop codon of ACTB gene.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: RcoRV (destroyed during cloning)
• 3′ cloning site: RcoRV (destroyed during cloning)
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: AATTAACCCTCACTAAAGG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry