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pAAV.hSynapsin1.OxLight-ctr
(Plasmid #169793)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169793 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV.hSynapsin1
  • Backbone manufacturer
    Viral Vector Facility - University of Zurich
  • Backbone size w/o insert (bp) 4438
  • Total vector size (bp) 6481
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OxLight-ctr
  • Species
    Synthetic
  • Insert Size (bp)
    2043
  • Mutation
    E54K; T111A
  • GenBank ID
    MW845971
  • Promoter human Synapsin-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer gtcgagattaattaactcgagcaggtaag
  • 3′ sequencing primer gcaaccaggatttatacaaggaggagaaaat
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.hSynapsin1.OxLight-ctr was a gift from Tommaso Patriarchi (Addgene plasmid # 169793 ; http://n2t.net/addgene:169793 ; RRID:Addgene_169793)

  • For your References section:

    A genetically encoded sensor for in vivo imaging of orexin neuropeptides. Duffet L, Kosar S, Panniello M, Viberti B, Bracey E, Zych AD, Radoux-Mergault A, Zhou X, Dernic J, Ravotto L, Tsai YC, Figueiredo M, Tyagarajan SK, Weber B, Stoeber M, Gogolla N, Schmidt MH, Adamantidis AR, Fellin T, Burdakov D, Patriarchi T. Nat Methods. 2022 Feb;19(2):231-241. doi: 10.1038/s41592-021-01390-2. Epub 2022 Feb 10. 10.1038/s41592-021-01390-2 PubMed 35145320
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