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Addgene

XP11 Goosecoid
(Plasmid #16979)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Goosecoid
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    850
  • Entrez Gene
    gsc-a (a.k.a. Xgsc, goosecoid)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII/BamHI (not destroyed)
  • 3′ cloning site BglII/BamHI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: coding region of Xenopus goosecoid in SP64T in sense (#9) & antisense (#2) orientation.

Transcription: linearize with EcoR1 and transcribe with SP6.

Plasmid History:
Constructed by Jan Christian by PCR.

Goosecoid cloned and characterized by lab of E. DeRobertis, UCLA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XP11 Goosecoid was a gift from Randall Moon (Addgene plasmid # 16979 ; http://n2t.net/addgene:16979 ; RRID:Addgene_16979)
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