pGRNA5e
(Plasmid
#169786)
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Purpose(Empty Backbone) Insert one gRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169786 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAll in One™ vector
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Backbone manufacturerBIOFACT
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGRNA5e was a gift from Sang-Gyu Kim (Addgene plasmid # 169786 ; http://n2t.net/addgene:169786 ; RRID:Addgene_169786) -
For your References section:
A multiplex guide RNA expression system and its efficacy for plant genome engineering. Oh Y, Lee B, Kim H, Kim SG. Plant Methods. 2020 Mar 12;16:37. doi: 10.1186/s13007-020-00580-x. eCollection 2020. 10.1186/s13007-020-00580-x PubMed 32190101