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Addgene

XP12 Xbra
(Plasmid #16978)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16978 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psp73
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xbra
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2200
  • Entrez Gene
    t.S (a.k.a. XELAEV_18029080mg, X-bra, Xbrachyury, bra, brachyury, bu, ntl, t, t-a, t-b, t.L, ta, xbra)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: full length Xbra from Jim Smith.

Antisense: linearize with EcoRV, transcribe with T7.

Plasmid History:
Constructed by Jan Christian.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XP12 Xbra was a gift from Randall Moon (Addgene plasmid # 16978 ; http://n2t.net/addgene:16978 ; RRID:Addgene_16978)
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