pECO300
(Plasmid
#169766)
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Purpose(Empty Backbone) Express SpCas9 and Kanamycin resistance gene
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169766 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHAtC
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Vector typePlant Expression ; Plant Binary vector
- Promoter 35S, AtU6
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pECO300 was a gift from Sang-Gyu Kim (Addgene plasmid # 169766 ; http://n2t.net/addgene:169766 ; RRID:Addgene_169766) -
For your References section:
A multiplex guide RNA expression system and its efficacy for plant genome engineering. Oh Y, Lee B, Kim H, Kim SG. Plant Methods. 2020 Mar 12;16:37. doi: 10.1186/s13007-020-00580-x. eCollection 2020. 10.1186/s13007-020-00580-x PubMed 32190101
Map uploaded by the depositor.
