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Addgene

GB1-PRD(703-868, P801G, T805C, C813S)-Strep
(Plasmid #169749)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169749 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Backbone size w/o insert (bp) 5643
  • Total vector size (bp) 6441
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep
  • Alt name
    ALIX Proline Rich Domain
  • Alt name
    ALIX Proline Rich Region
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    798
  • Mutation
    Proline 801 to Glycine, Threonine 805 to Cysteine, Cysteine 813 to Serine
  • Entrez Gene
    PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
  • Tags / Fusion Proteins
    • GB1-His6-TEV (N terminal on insert)
    • Strep tag (WSHPQFEK) (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GB1-PRD(703-868, P801G, T805C, C813S)-Strep was a gift from Lalit Deshmukh (Addgene plasmid # 169749 ; http://n2t.net/addgene:169749 ; RRID:Addgene_169749)

  • For your References section:

    Mechanistic Roles of Tyrosine Phosphorylation in Reversible Amyloids, Autoinhibition, and Endosomal Membrane Association of ALIX. Elias RD, Ramaraju B, Deshmukh L. J Biol Chem. 2021 Oct 21:101328. doi: 10.1016/j.jbc.2021.101328. 10.1016/j.jbc.2021.101328 PubMed 34688656
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