pDEST17-cDAXX-0SB
(Plasmid
#169730)
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PurposeBacterial expression of N-terminally 6His tagged cDAXX-0SB
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169730 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST17
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Backbone manufacturerInvitrogen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecDAXX-0SB
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SpeciesH. sapiens (human)
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Insert Size (bp)732
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MutationComprises residues 495-741 and has the major SB motifs mutated
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Entrez GeneDAXX (a.k.a. BING2, DAP6, EAP1, SMIM40)
- Promoter T7
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Tag
/ Fusion Protein
- 6 His (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST17-cDAXX-0SB was a gift from Tanja Mittag (Addgene plasmid # 169730 ; http://n2t.net/addgene:169730 ; RRID:Addgene_169730) -
For your References section:
Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation of Active, Phase-Separated Compartments. Bouchard JJ, Otero JH, Scott DC, Szulc E, Martin EW, Sabri N, Granata D, Marzahn MR, Lindorff-Larsen K, Salvatella X, Schulman BA, Mittag T. Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi: 10.1016/j.molcel.2018.08.027. Epub 2018 Sep 20. 10.1016/j.molcel.2018.08.027 PubMed 30244836