XP31 Vox
(Plasmid
#16972)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16972 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVox
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Alt nameVox-15
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Alt nameVent-2
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SpeciesX. laevis (frog)
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Entrez Geneventx2.1.L (a.k.a. XELAEV_18034617mg, Xbr-1a, Xvr-11b, ventx2.1-a, ventx2.1-b, vox, vox1, xom, xvent-2, xvent-2b)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Transcription: antisense probe is made by digesting with BamHI and transcribing with T7.
Plasmid History:
Constructed by Jennifer Schmidt in 1996.
Ref: Schmidt et al, 1996, Development 122:1711-1721.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XP31 Vox was a gift from Randall Moon (Addgene plasmid # 16972 ; http://n2t.net/addgene:16972 ; RRID:Addgene_16972) -
For your References section:
Regulation of dorsal-ventral patterning: the ventralizing effects of the novel Xenopus homeobox gene Vox. Schmidt JE, von Dassow G, Kimelman D. Development. 1996 Jun;122(6):1711-21. doi: 10.1242/dev.122.6.1711. 10.1242/dev.122.6.1711 PubMed 8674411