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Addgene

A1-LCD Aro--
(Plasmid #169716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169716 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST17
  • Backbone manufacturer
    invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hnRNPA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    495
  • Mutation
    hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. Serine mutations at residues: 203, 223, 255, 261, 297, 315
  • Entrez Gene
    HNRNPA1 (a.k.a. ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, MPD3, UP 1, hnRNP A1, hnRNP-A1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    A1-LCD Aro-- was a gift from Tanja Mittag (Addgene plasmid # 169716 ; http://n2t.net/addgene:169716 ; RRID:Addgene_169716)

  • For your References section:

    Valence and patterning of aromatic residues determine the phase behavior of prion-like domains. Martin EW, Holehouse AS, Peran I, Farag M, Incicco JJ, Bremer A, Grace CR, Soranno A, Pappu RV, Mittag T. Science. 2020 Feb 7;367(6478):694-699. doi: 10.1126/science.aaw8653. 10.1126/science.aaw8653 PubMed 32029630
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