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Addgene

Ash1-5p
(Plasmid #169712)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169712 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST17
  • Backbone manufacturer
    invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ash1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    327
  • Mutation
    Truncation including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed
  • Entrez Gene
    ASH1 (a.k.a. YKL185W)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ash1-5p was a gift from Tanja Mittag (Addgene plasmid # 169712 ; http://n2t.net/addgene:169712 ; RRID:Addgene_169712)

  • For your References section:

    Sequence Determinants of the Conformational Properties of an Intrinsically Disordered Protein Prior to and upon Multisite Phosphorylation. Martin EW, Holehouse AS, Grace CR, Hughes A, Pappu RV, Mittag T. J Am Chem Soc. 2016 Nov 30;138(47):15323-15335. doi: 10.1021/jacs.6b10272. Epub 2016 Nov 17. 10.1021/jacs.6b10272 PubMed 27807972
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