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Addgene

pGBK CUP1 Empty
(Plasmid #169710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Modified pGBKT7
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 7027
  • Modifications to backbone
    Replaced the ADH1 promoter with a full length modified CUP1 promoter (modified the BamHI and NdeI site).
  • Vector type
    Bacterial Expression, Yeast Expression
  • Promoter CUP1
  • Selectable markers
    TRP1
  • Tags / Fusion Proteins
    • GAL4 DNA Binding Domain (N terminal on backbone)
    • Myc (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBK CUP1 Empty was a gift from Bruce Hamilton (Addgene plasmid # 169710 ; http://n2t.net/addgene:169710 ; RRID:Addgene_169710)

  • For your References section:

    Inducible Yeast Two-Hybrid with Quantitative Measures. Hernandez J, Ross K, Hamilton B. bioRxiv 10.1101/2021.07.01.450807
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