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rAAV-U6-MeCP2shRNA-CamKIIa-mCherry
(Plasmid #169704)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA against MeCp2
  • gRNA/shRNA sequence
    GTCAGAAGACCAGGATCTC
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mecp2 (a.k.a. 1500041B07Rik, D630021H01Rik, Mbd5, WBP10)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the 11bp deletion in ITR and F75S mutation in mCherry do not alter plasmid function. Also cited in https://pubmed.ncbi.nlm.nih.gov/33203444/.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rAAV-U6-MeCP2shRNA-CamKIIa-mCherry was a gift from Ana Oliveira (Addgene plasmid # 169704 ; http://n2t.net/addgene:169704 ; RRID:Addgene_169704)

  • For your References section:

    Adult hippocampal MeCP2 preserves the genomic responsiveness to learning required for long-term memory formation. Gulmez Karaca K, Brito DVC, Zeuch B, Oliveira AMM. Neurobiol Learn Mem. 2018 Mar;149:84-97. doi: 10.1016/j.nlm.2018.02.010. Epub 2018 Feb 10. 10.1016/j.nlm.2018.02.010 PubMed 29438740
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