pBRC_double_attB_GFP_donor
(Plasmid
#169692)
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Purpose(Empty Backbone) cargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPD158.87
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Backbone manufacturerAndrew Fire
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Modifications to backboneadd two inwardly facing phiC31 attB sites add Cbr_unc-119 rescue gene add Gateway R1R2
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Vector typeWorm Expression
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Selectable markersPsur5:GFP and Cbr_unc-119
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2020.11.25.398784 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBRC_double_attB_GFP_donor was a gift from John Wang (Addgene plasmid # 169692 ; http://n2t.net/addgene:169692 ; RRID:Addgene_169692) -
For your References section:
phiC31 integrase for recombination-mediated single-copy insertion and genome manipulation in Caenorhabditis elegans. Yang FJ, Chen CN, Chang T, Cheng TW, Chang NC, Kao CY, Lee CC, Huang YC, Hsu JC, Li J, Lu MJ, Chan SP, Wang J. Genetics. 2022 Feb 4;220(2):iyab206. doi: 10.1093/genetics/iyab206. 10.1093/genetics/iyab206 PubMed 34791215
Map uploaded by the depositor.
