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pBRC_double_attB_GFP_donor
(Plasmid #169692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD158.87
  • Backbone manufacturer
    Andrew Fire
  • Modifications to backbone
    add two inwardly facing phiC31 attB sites add Cbr_unc-119 rescue gene add Gateway R1R2
  • Vector type
    Worm Expression
  • Selectable markers
    Psur5:GFP and Cbr_unc-119

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2020.11.25.398784 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBRC_double_attB_GFP_donor was a gift from John Wang (Addgene plasmid # 169692 ; http://n2t.net/addgene:169692 ; RRID:Addgene_169692)

  • For your References section:

    phiC31 integrase for recombination-mediated single-copy insertion and genome manipulation in Caenorhabditis elegans. Yang FJ, Chen CN, Chang T, Cheng TW, Chang NC, Kao CY, Lee CC, Huang YC, Hsu JC, Li J, Lu MJ, Chan SP, Wang J. Genetics. 2022 Feb 4;220(2):iyab206. doi: 10.1093/genetics/iyab206. 10.1093/genetics/iyab206 PubMed 34791215
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