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Addgene

EGFP–acVHH–PB
(Plasmid #169685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169685 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mEGFP-C1
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-caffeine nanobody
  • Species
    Synthetic
  • Insert Size (bp)
    354
  • Promoter CMV
  • Tag / Fusion Protein
    • modified STIM1-PB tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFPC1F: GAT CAC TCT CGG CAT GGA C
  • 3′ sequencing primer SV40pA-R: GAA ATT TGT GAT GCT ATT GC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP–acVHH–PB was a gift from Yubin Zhou (Addgene plasmid # 169685 ; http://n2t.net/addgene:169685 ; RRID:Addgene_169685)

  • For your References section:

    Caffeine-Operated Synthetic Modules for Chemogenetic Control of Protein Activities by Life Style. Wang T, He L, Jing J, Lan TH, Hong T, Wang F, Huang Y, Ma G, Zhou Y. Adv Sci (Weinh). 2020 Dec 13;8(3):2002148. doi: 10.1002/advs.202002148. eCollection 2021 Feb. 10.1002/advs.202002148 PubMed 33552855
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