pTS2317
(Plasmid #169624)

• Purpose: Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
• Depositing Lab: Martin Fussenegger
• Publication: Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.1016/j.ymben.2021.04.003. Epub 2021 Apr 18.

Backbone

• Vector backbone: pVH1002 (pUC57-based)
• Backbone size w/o insert (bp): 2689
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
• Alt name: PCMV-SEAP-p2a-iRFP::A2::pGL3-PPGK-IgkSS-Nluc-p2a-mTagBFP2
• Species: H. sapiens (human); Synthetic
• Promoter: PhCMV / PPGK

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: AmpStart
• 3′ sequencing primer: CTGGCACGACAGGTTTCCCGACTGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry