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Addgene

pBluescript Engrailed-2
(Plasmid #16950)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Xenopus Probe

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Engrailed-2
  • Alt name
    En-2
  • Alt name
    En2
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1400
  • Entrez Gene
    en2-b (a.k.a. En2, eng2, xen2, maben, engrailed-2)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In situ probe cut with Xba1, transcribe with T3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript Engrailed-2 was a gift from Richard Harland (Addgene plasmid # 16950 ; http://n2t.net/addgene:16950 ; RRID:Addgene_16950)

  • For your References section:

    Cephalic expression and molecular characterization of Xenopus En-2. Hemmati-Brivanlou A, de la Torre JR, Holt C, Harland RM. Development. 1991 Mar . 111(3):715-24. PubMed 1679005
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