pBluescript Engrailed-2
(Plasmid
#16950)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16950 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeXenopus Probe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEngrailed-2
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Alt nameEn-2
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Alt nameEn2
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SpeciesX. laevis (frog)
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Insert Size (bp)1400
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Entrez Geneen2-b (a.k.a. En2, eng2, xen2, maben, engrailed-2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In situ probe cut with Xba1, transcribe with T3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBluescript Engrailed-2 was a gift from Richard Harland (Addgene plasmid # 16950 ; http://n2t.net/addgene:16950 ; RRID:Addgene_16950) -
For your References section:
Cephalic expression and molecular characterization of Xenopus En-2. Hemmati-Brivanlou A, de la Torre JR, Holt C, Harland RM. Development. 1991 Mar . 111(3):715-24. PubMed 1679005
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/42/16/1dba866c-af62-11e0-90fe-003048dd6500.gif.940x940_q85_autocrop.png)