mGFAP R236H
(Plasmid
#169475)
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PurposeSingle p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHaloTAG mGFAP (R236H)
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SpeciesM. musculus (mouse)
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MutationChanged R 236 to H
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Entrez GeneGfap
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Tag
/ Fusion Protein
- EGFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mGFAP R236H was a gift from Federico Herrera (Addgene plasmid # 169475 ; http://n2t.net/addgene:169475 ; RRID:Addgene_169475) -
For your References section:
New tools for the visualization of glial fibrillary acidicprotein in living cells. Letra-Vilela R, Quiteres R, Murtinheira F, Crevenna A, Hensel Z, Herrera F. Experimental Results (2020), Volume 1, e4, 1–8 10.1017/exp.2020.1