mGFAP-Halo
(Plasmid #169474)

• Purpose: the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites
• Depositing Lab: Federico Herrera
• Publication: Letra-Vilela et al Experimental Results (2020), Volume 1, e4, 1–8

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFAP
• Species: M. musculus (mouse)
• Mutation: Changed R 236 to H
• Entrez Gene: Gfap
• Tag / Fusion Protein:
  • HaloTag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry