pTwist-SARS-CoV Δ18
(Plasmid
#169465)
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PurposeEncodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTwist
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSARS-CoV Spike truncated to remove 18 amino acids
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Alt nameSARS-CoV-1 spike
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Entrez GeneS (a.k.a. sars2, E2)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTwist-SARS-CoV Δ18 was a gift from Alejandro Balazs (Addgene plasmid # 169465 ; http://n2t.net/addgene:169465 ; RRID:Addgene_169465) -
For your References section:
Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity. Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB. Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 10.1016/j.cell.2021.03.013. 10.1016/j.cell.2021.03.013 PubMed 33743213
