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Addgene

pDisplay-ZIBG2
(Plasmid #169458)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169458 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDisplay
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZIBG2
  • Species
    Synthetic
  • Insert Size (bp)
    825
  • GenBank ID
    MK673552
  • Tag / Fusion Protein
    • pDisplay backbone elements for export and membrane anchor

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

When selecting zinc biosensors for your particular application, please pay attention to the Kd. The Kd of the sensor has to match the concentration range of your particular experiments.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-ZIBG2 was a gift from Huiwang Ai (Addgene plasmid # 169458 ; http://n2t.net/addgene:169458 ; RRID:Addgene_169458)
  • For your References section:

    Genetically Encoded, Photostable Indicators to Image Dynamic Zn(2+) Secretion of Pancreatic Islets. Chen M, Zhang S, Xing Y, Li X, He Y, Wang Y, Oberholzer J, Ai HW. Anal Chem. 2019 Oct 1;91(19):12212-12219. doi: 10.1021/acs.analchem.9b01802. Epub 2019 Sep 10. 10.1021/acs.analchem.9b01802 PubMed 31475537