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PurposeExpression of 2 guides + donor DNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169442 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSuper ecliptic pHluorin (SEP)
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gRNA/shRNA sequenceUnknown
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-SEP-GluA2 TKIT was a gift from Richard Huganir (Addgene plasmid # 169442 ; http://n2t.net/addgene:169442 ; RRID:Addgene_169442) -
For your References section:
An optimized CRISPR/Cas9 approach for precise genome editing in neurons. Fang H, Bygrave AM, Roth RH, Johnson RC, Huganir RL. Elife. 2021 Mar 10;10. pii: 65202. doi: 10.7554/eLife.65202. 10.7554/eLife.65202 PubMed 33689678