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Addgene

pHFS:SEP-rGluA1 TKIT donor
(Plasmid #169438)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169438 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHFS
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Super ecliptic pHluorin (SEP)
  • Species
    R. norvegicus (rat)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHFS:SEP-rGluA1 TKIT donor was a gift from Richard Huganir (Addgene plasmid # 169438 ; http://n2t.net/addgene:169438 ; RRID:Addgene_169438)

  • For your References section:

    An optimized CRISPR/Cas9 approach for precise genome editing in neurons. Fang H, Bygrave AM, Roth RH, Johnson RC, Huganir RL. Elife. 2021 Mar 10;10. pii: 65202. doi: 10.7554/eLife.65202. 10.7554/eLife.65202 PubMed 33689678
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