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Addgene

GluN2B (px330:2-guideCas9)
(Plasmid #169430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spCas9
  • gRNA/shRNA sequence
    Unknown
  • Species
    M. musculus (mouse)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GluN2B (px330:2-guideCas9) was a gift from Richard Huganir (Addgene plasmid # 169430 ; http://n2t.net/addgene:169430 ; RRID:Addgene_169430)

  • For your References section:

    An optimized CRISPR/Cas9 approach for precise genome editing in neurons. Fang H, Bygrave AM, Roth RH, Johnson RC, Huganir RL. Elife. 2021 Mar 10;10. pii: 65202. doi: 10.7554/eLife.65202. 10.7554/eLife.65202 PubMed 33689678
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