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pAAV-6xTRE-FLEX-hM4Di.mCherry
(Plasmid #169415)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169415 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hM4Di.mCherry
  • Species
    Synthetic
  • Mutation
    hM4Di is Gi-coupled DREADD.Derived from human muscarinic receptor M4 sequence with two point mutations (Y113C,A203G)
  • Promoter Minimal TATA
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modified Tet operator repeats. Construct might show tTA-independent "leaky" expression in some cell types.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-6xTRE-FLEX-hM4Di.mCherry was a gift from David Talmage (Addgene plasmid # 169415 ; http://n2t.net/addgene:169415 ; RRID:Addgene_169415)

  • For your References section:

    Functionally refined encoding of threat memory by distinct populations of basal forebrain cholinergic projection neurons. Rajebhosale P, Ananth MR, Kim R, Crouse RB, Jiang L, Lopez-Hernandez G, Zhong C, Arty C, Wang S, Jone A, Desai NS, Li Y, Picciotto MR, Role LW, Talmage DA. Elife. 2024 Feb 16;13:e86581. doi: 10.7554/eLife.86581. 10.7554/eLife.86581 PubMed 38363713
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