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PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMV306
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namepLeft
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Insert Size (bp)400
Gene/Insert 2
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Gene/Insert namemScarlet proper
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Insert Size (bp)692
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Plasmid L5 attB::Pleft* mScarlet was a gift from Clifton Barry III (Addgene plasmid # 169410 ; http://n2t.net/addgene:169410 ; RRID:Addgene_169410) -
For your References section:
Development and Optimization of Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs. Kolbe K, Bell AC, Prosser GA, Assmann M, Yang HJ, Forbes HE, Gallucci S, Mayer-Barber KD, Boshoff HI, Barry Iii CE. Front Microbiol. 2020 Dec 9;11:591866. doi: 10.3389/fmicb.2020.591866. eCollection 2020. 10.3389/fmicb.2020.591866 PubMed 33362741