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Addgene

ZP1 Wnt-8
(Plasmid #16941)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16941 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGem-4
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wnt-8
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    600
  • Entrez Gene
    wnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: RACE'd wnt-8. Wnt-8 in situ clone 25. Sense probe: cut with XbaI, transcribe with SP6.
Antisense probe: cut with EcoR1, transcribe with T7.

Plasmid History:
Constructed by Greg Kelly, 10/93.

Notes for Use:
Long 5' end of wnt-8 good in situ probe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP1 Wnt-8 was a gift from Randall Moon (Addgene plasmid # 16941 ; http://n2t.net/addgene:16941 ; RRID:Addgene_16941)
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