pmCellFree_KA1_SARS-COV-2_RBD -N501Y
(Plasmid
#169407)
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PurposeCell-free/mammalian expression vector for SARS-COV-2 mutated RBD domain (N510Y, UK strain) of S protein with N-term eGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169407 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepmCellFree_KA3
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Vector typeCell-free expression vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-COV-2 _RBD (N501Y)
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SpeciesSARS-Cov2
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MutationN501Y
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
- Promoter T7- CMV/CAG
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Tag
/ Fusion Protein
- His and eGFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCellFree_KA1_SARS-COV-2_RBD -N501Y was a gift from Kirill Alexandrov (Addgene plasmid # 169407 ; http://n2t.net/addgene:169407 ; RRID:Addgene_169407)
