pmCell-Free_KA1_SARS-COV-2_S-frag3
(Plasmid
#169403)
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PurposeCell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169403 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCellFree_KA1
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Vector typeCell-free expression vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN-term frag. S protein after the furin cleavag
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SpeciesSARS-Cov2
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Entrez GeneN (a.k.a. GU280_gp10)
- Promoter T7- CMV/CAG
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Tag
/ Fusion Protein
- His and eGFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCell-Free_KA1_SARS-COV-2_S-frag3 was a gift from Kirill Alexandrov (Addgene plasmid # 169403 ; http://n2t.net/addgene:169403 ; RRID:Addgene_169403)