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Addgene

pmCellFree_KA1_SARS-COV-2_ORF7b
(Plasmid #169394)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCellFree_KA1
  • Vector type
    Cell-free expression vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-COV-2 _ORF7b
  • Species
    SARS-Cov2
  • Mutation
    Codon optimized for Saccharomyces cerevisiae
  • Entrez Gene
    ORF7b (a.k.a. GU280_gp08)
  • Promoter T7- CMV/CAG
  • Tag / Fusion Protein
    • His and eGFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCellFree_KA1_SARS-COV-2_ORF7b was a gift from Kirill Alexandrov (Addgene plasmid # 169394 ; http://n2t.net/addgene:169394 ; RRID:Addgene_169394)
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