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Addgene

pBS-tWnt7b
(Plasmid #169371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescriptSK
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wnt7b
  • Species
    Chinese soft-shell turtle
  • Insert Size (bp)
    1000
  • GenBank ID
    ENSPSIG00000018199 (Ensemble)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer M13 fwd
  • 3′ sequencing primer M13 rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-tWnt7b was a gift from Katsuhiko Ono (Addgene plasmid # 169371 ; http://n2t.net/addgene:169371 ; RRID:Addgene_169371)

  • For your References section:

    Changes in Wnt-Dependent Neuronal Morphology Underlie the Anatomical Diversification of Neocortical Homologs in Amniotes. Nomura T, Ohtaka-Maruyama C, Kiyonari H, Gotoh H, Ono K. Cell Rep. 2020 May 5;31(5):107592. doi: 10.1016/j.celrep.2020.107592. 10.1016/j.celrep.2020.107592 PubMed 32375034
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