pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
(Plasmid
#169369)
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PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoes
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169369 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR2.1
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Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 3931
- Total vector size (bp) 4819
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
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Alt nameCPIJ039653
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SpeciesCulex quinquefasciatus
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Insert Size (bp)888
- Promoter CPIJ039653
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop was a gift from Valentino Gantz (Addgene plasmid # 169369 ; http://n2t.net/addgene:169369 ; RRID:Addgene_169369) -
For your References section:
Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes. Feng X, Lopez Del Amo V, Mameli E, Lee M, Bishop AL, Perrimon N, Gantz VM. Nat Commun. 2021 May 20;12(1):2960. doi: 10.1038/s41467-021-23239-0. 10.1038/s41467-021-23239-0 PubMed 34017003
Map uploaded by the depositor.
