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Addgene

pDC146
(Plasmid #169366)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet
  • Total vector size (bp) 7801
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
  • Species
    R. norvegicus (rat)
  • Promoter T7
  • Tag / Fusion Protein
    • 6 his tag

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Daniel Cipriano

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDC146 was a gift from Axel Brunger (Addgene plasmid # 169366 ; http://n2t.net/addgene:169366 ; RRID:Addgene_169366)

  • For your References section:

    Processive ATP-driven substrate disassembly by the N-ethylmaleimide-sensitive factor (NSF) molecular machine. Cipriano DJ, Jung J, Vivona S, Fenn TD, Brunger AT, Bryant Z. J Biol Chem. 2013 Aug 9;288(32):23436-45. doi: 10.1074/jbc.M113.476705. Epub 2013 Jun 17. 10.1074/jbc.M113.476705 PubMed 23775070
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