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Addgene

pBluescript goosecoid
(Plasmid #16935)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    goosecoid
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1200
  • Entrez Gene
    gsc (a.k.a. ZGSC, ik:tdsubc_1c10, ik:tdsubc_2g4, ik:tdsubc_2h11, xx:tdsubc_1c10, xx:tdsubc_2g4, xx:tdsubc_2h11, zgc:101595)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Antisense probe cut with EcoR1 and transcribe with T7.
Sense probe cut with Xho1 and transcribe with T3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript goosecoid was a gift from John Postlethwait (Addgene plasmid # 16935 ; http://n2t.net/addgene:16935 ; RRID:Addgene_16935)

  • For your References section:

    Goosecoid expression in neurectoderm and mesendoderm is disrupted in zebrafish cyclops gastrulas. Thisse C, Thisse B, Halpern ME, Postlethwait JH. Dev Biol. 1994 Aug . 164(2):420-9. 10.1006/dbio.1994.1212 PubMed 8045345
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