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pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-
(Plasmid #169338)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169338 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA5/FRT
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fucci(CA)
  • Species
    H. sapiens (human)
  • Mutation
    HaloTag9 = HaloTag7-Q165H-P174R
  • Entrez Gene
    GMNN (a.k.a. Gem, MGORS6)
  • Entrez Gene
    CDT1 (a.k.a. DUP, RIS2)
  • Tags / Fusion Proteins
    • HaloTag7-Geminin(1/110) (N terminal on insert)
    • HaloTag9-Cdt(1-110)Cy- (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy- was a gift from Kai Johnsson (Addgene plasmid # 169338 ; http://n2t.net/addgene:169338 ; RRID:Addgene_169338)

  • For your References section:

    Engineered HaloTag variants for fluorescence lifetime multiplexing. Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K. Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x. 10.1038/s41592-021-01341-x PubMed 34916672
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